35 research outputs found
GATA3 is a sensitive marker for primary genital extramammary paget disease: an immunohistochemical study of 72 cases with comparison to gross cystic disease fluid protein 15
Dexamethasone for the Prophylaxis of Radiation-Induced Emesis: A National Cancer Institute of Canada Clinical Trials Group Phase III Study
Randomized trial of a chlorhexidine mouthwash for alleviation of radiation-induced mucositis.
Theopederins K and L. Highly Potent Cytotoxic Metabolites from a Marine Sponge Discodermia
Abbreviated Course of Radiation Therapy in Older Patients With Glioblastoma Multiforme: A Prospective Randomized Clinical Trial
Microspinosamide, a New HIV-Inhibitory Cyclic Depsipeptide from the Marine Sponge Sidonops
Nobiloside, a New Neuraminidase Inhibitory Triterpenoidal Saponin from the Marine Sponge Erylus nobilis
Antimicrobial activity of heterotrophic bacterial communities from the marine sponge Erylus discophorus (Astrophorida, Geodiidae)
10 páginas, 3 tablas.Heterotrophic bacteria associated with two specimens of the marine sponge Erylus discophorus were screened for their
capacity to produce bioactive compounds against a panel of human pathogens (Staphylococcus aureus wild type and
methicillin-resistant S. aureus (MRSA), Bacillus subtilis, Pseudomonas aeruginosa, Acinetobacter baumanii, Candida albicans
and Aspergillus fumigatus), fish pathogen (Aliivibrio fischeri) and environmentally relevant bacteria (Vibrio harveyi). The
sponges were collected in Berlengas Islands, Portugal. Of the 212 isolated heterotrophic bacteria belonging to Alpha- and
Gammaproteobacteria, Actinobacteria and Firmicutes, 31% produced antimicrobial metabolites. Bioactivity was found
against both Gram positive and Gram negative and clinically and environmentally relevant target microorganisms.
Bioactivity was found mainly against B. subtilis and some bioactivity against S. aureus MRSA, V. harveyi and A. fisheri. No
antifungal activity was detected. The three most bioactive genera were Pseudovibrio (47.0%), Vibrio (22.7%) and Bacillus
(7.6%). Other less bioactive genera were Labrenzia, Acinetobacter, Microbulbifer, Pseudomonas, Gordonia, Microbacterium,
Micrococcus and Mycobacterium, Paenibacillus and Staphylococcus. The search of polyketide I synthases (PKS-I) and
nonribosomal peptide synthetases (NRPSs) genes in 59 of the bioactive bacteria suggested the presence of PKS-I in 12
strains, NRPS in 3 strains and both genes in 3 strains. Our results show the potential of the bacterial community associated
with Erylus discophorus sponges as producers of bioactive compounds.This research was supported by the European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Programme
and national funds through FCT – Foundation for Science and Technology, under the projects PEst-C/MAR/LA0015/2013, PEst-OE/QUI/UI0612/2011 and PTDC/
QUI-QUI/098053/2008. Ana PatrĂcia Graçaa acknowledges an ERAMUS fellowship. Joana Bondoso was financed by FCT - Foundation for Science and Technology
(PhD grant SFRH/BD/35933/2007). Joana R. Xavier’s research is funded by an FCT - Foundation for Science and Technology postdoctoral fellowship (grant
no. SFRH/BPD/62946/2009). In addition, this work was funded by the Fundación MEDINA, a public-private partnership of Merck Sharp and Dohme of España/
Universidad de Granada/Junta de AndalucĂaPeer reviewe
New 9-thiocyanatopupukeanane sesquiterpenes from the nudibranch Phyllidia varicosa and its sponge-prey Axinyssa aculeata
Two new 9-thiocyanatopupukeanane sesquiterpene isomers were isolated as major metabolites from the MeOH extract of the sponge Axinyssa aculeata and from its nudibranch predator Phyllidia varicosa. The presence of the sesquiterpenes was monitored by GC-MS, and the structures were confirmed by both 1D and 2D NMR spectroscopy. The isolated sesquiterpenes were found to be toxic toward brine shrimp at LC(50) of 5 ppm. At a dose level of 20 microg, they were found to be weakly and moderately active against B. subtilis and C. albicans, respectively